Detection of causative agents of bacterial pneumonia in hospitalized hajj and umrah cases by multiplex real-time polymerase chain reaction

Detection of causative agents of bacterial pneumonia in hospitalized hajj and umrah cases by multiplex real-time polymerase chain reaction

Blog Article

Background and Objectives: Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is commonly detected in pneumonia patients who travel from the Middle East regions.Besides MERS-CoV, many other pathogenic agents cause pneumonia.Detection of such organisms must be done swiftly, especially in case of the negative MERS-CoV samples.The aim of this study was to identify the pathogenic agents that might account for bacterial pneumonia, from Hajj and Umrah pneumonia cases.Materials and Methods: We conducted a cross-sectional study, 38 pneumonia clinical samples from suffering of Mushroom Vapes Hajj and Umrah in 2017 with negative MERS-CoV were selected.

The laboratory testing was done at National Reference Laboratory in Jakarta and performed by multiplex real-time PCR using a FTD respiratory pathogens.Results: Haemophilus influenzae (26.4%) was the most frequent bacteria detected.Other causative agents of bacterial pneumonia identified were Moraxella catarrhalis (20.8%), Klebsiella pneumoniae (13.

2%), Streptococcus pneumoniae (9.4%), and Staphylococcus aureus (5.7%).From 38 samples showed that 25 (65.79%) samples were positive with bacteria, including five samples with coinfection.

The coinfection were combinations among S.aureus and S.pneumoniae (1/20), S.pneumoniae and K.pneumoniae (1/20), S.

pneumoniae and M.catarrhalis (2/20), S.pneumoniae and H.influenzae (2/20), K.pneumoniae and H.

influenzae (5/20), and M.catarrhalis Spoons and H.influenzae (5/20).Conclusion: Haemophilus influenzae is the most recurrent bacteria to be identified in samples of pneumonia of hajj and umrah cases.